Journal: Toxins

Article Title: Co-Expression and Co-Purification Enable Manufacturing of a Six-Monoclonal Antibody Botulinum Antitoxin Cocktail

doi: 10.3390/toxins18050199

Figure Lengend Snippet: IEX HPLC Analysis of 5 mL HiTrap Capto S Column Fraction 15 (blue) and S column load (black). The inset shows the protein concentration measured by A280 on the Nanodrop spectrophotometer for the collected peak fractions (1 mL per fraction). Fraction 15 in the histogram is highlighted in blue. (IEX-HPLC of individual purified antibodies is shown in .) The column was loaded with 20 μg antibody.

Article Snippet: Cytiva HiTrap Capto MMC, Cytiva MabSelect PrismA, Cytiva Fibro PrismA, Eshmuno CP-FT, Cytiva Capto Q (1 mL), Cytiva Capto S (1 mL), Cytiva Capto Adhere (1 mL), MabSelect SuRe resin, MilliporeSigma ViResolve Modus 1.1 viral reduction filter (Sigma, St. Louis, MO, USA), Pall (Port Washington, NY, USA) Cadence Omega 0.1 m 2 30 kDa TFF cassette.

Techniques: Protein Concentration, Spectrophotometry, Purification

Journal: Journal of Biological Methods

Article Title: Efficient removal of antibody aggregates using TOYOPEARL MX-Trp-650M mixed-mode resin under salt or pH gradient elution

doi: 10.14440/jbm.0029

Figure Lengend Snippet: Capto MMC ImpRes run data obtained under linear salt gradient elution. (A) Capto MMC ImpRes chromatogram of a run conducted under linear salt gradient elution. The load material was Protein A eluate, which contained 15.3% of aggregates. (B) SEC-HPLC profile of a sample aliquot from the Capto MMC ImpRes elution pool. The percentages of monomer and aggregates are labeled. Notes: Inset: Native gel analysis of relevant fractions. L: Load material; Lanes 1 – 3: Elution fractions 1 – 3; S: Strip. Bands corresponding to monomeric and aggregated antibodies are indicated. The main elution fraction contained two bands, which likely represent glycosylation isoforms, the same reason that led to peak splitting. Abbreviation: SEC-HPLC: Size-exclusion chromatography-high-performance liquid chromatography.

Article Snippet: Capto MMC ImpRes is a mixed-mode resin from Cytiva, and its ligand contains both cation exchange and hydrophobic moieties.

Techniques: Labeling, Stripping Membranes, Glycoproteomics, Size-exclusion Chromatography, High Performance Liquid Chromatography

Journal: Journal of Biological Methods

Article Title: Efficient removal of antibody aggregates using TOYOPEARL MX-Trp-650M mixed-mode resin under salt or pH gradient elution

doi: 10.14440/jbm.0029

Figure Lengend Snippet: The plot of K p -monomer and K p -aggregates under various salt concentrations (conductivities) using two different mixed-mode resins. (A) MX-Trp-650M, pH 4.5. (B) Capto MMC ImpRes, pH 5.5. For each resin, K p ratios under each salt concentration are given in the table within the corresponding figure.

Article Snippet: Capto MMC ImpRes is a mixed-mode resin from Cytiva, and its ligand contains both cation exchange and hydrophobic moieties.

Techniques: Concentration Assay